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The mRNA levels of CCL2 (C-C Motif Chemokine Ligand 2), CCL11 (C-C Motif Chemokine Ligand 11), CSF2 (Colony Stimulating Factor 2), CXCL10 (C-X-C Motif Chemokine Ligand 10), HGF (Hepatocyte Growth Factor), IL1RA (Interleukin 1 Receptor Antagonist), IL6 (Interleukin 6), IL10 (Interleukin 10), IL31 (Interleukin 31), LIF (LIF Interleukin 6 Family Cytokine), and NGF (Nerve Growth Factor) were quantified using specific primers in SYBR Green (Thermo Fisher) or TaqMan (Thermo Fisher) platforms following the manufacturers instructions
aureus were stained with 100300 nM LysoTracker Green DND-26 (ThermoFisher) at 37 C for 30 min, then washed and maintained in 200 l complete MEM containing 1 g ml 1 Hoechst 33342, 50 g ml 1 Gen and 2 M aTc
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Understanding the transport of GSH across the plasma membrane is an important issue that is essential to treatment of diseases involving oxidative stress (see reviews by Ballatori et al., 2008 and by Yuan and Kaplowitz, 2008 in this issue)
Dai S, Hirayama T, Abbas S, Abu-Amer Y