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Angle the needle so that the tip is resting against the inside glass wall of the vial

Serum acetylcholine (ACh) levels, reduced in the FC group, increased significantly in both BDF groups, with the BDF-H group showing a 20% rise (Figure 2E)

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doi: 10.1088/1758-5090/adc15 107 AlamMZDevalarajaSHaldarM

Circulation journal: official journal of the Japanese Circulation Society 79, 11451155 (2015)