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Exp Clin Endocrinol Diabetes 2005

10.1620/tjem.251.87 104 MiyataH.TakadaT.ToyodaY.MatsuoH.IchidaK.SuzukiH

All participants provided written informed consent in accordance with the Declaration of Helsinki

Sections were stained with primary antibodies against Ki67 (1:200

Processing of tissue samples and hemoglobin estimation Blood, 100 L of sample drawn by cardiac puncture under ether anesthesia (50 g/kg body weight) are centrifuged and washed twice with 5 mL of 0,9% NaCl