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Frozen slices 12 mm thick were blocked with PBS + 5% NGS, labeled with a Rat Monoclonal Antibody for CD54eBioKAT-1from eBioscience, Invitrogen diluted 1:200 in blocking buffer, then with an Alexa 488 Goat anti-Rat IgG secondary antibody diluted 1:500 in blocking buffer and counterstained with DAPI, and mounted in Vectashield mounting media for fluorescence

After 24 hours of incubation at 37C, colony counts were performed to quantify surviving bacteria

IGF-1 induces hypoxia-inducible factor 1-mediated GLUT3 expression through PI3K/Akt/mTOR dependent pathways in PC12 cells

In the Enhanced model, the human body that plays sport is a regulated product of a private company whose binding obligation is to its quarterly numbers

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