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Indeed, mutating Asp222 of diverse plant TyrA a into the corresponding Asn residue in legume TyrA p reduced their ADH activity and introduced PDH activity, suggesting that Asn222 played a key role in the recent evolution of legume-specific TyrA p (Schenck et al., 2017)
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Crystals were directly harvested from the initial screening plate and transferred to cryoprotectant Paratone-N oil (Hampton Research, Aliso Viejo, CA, USA) and flash-frozen with a liquid-nitrogen gas stream
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